ABSTRACT
Objective:To study the effect of Jieyu Qutan Huazhuo prescription（JQHP） on the gut microbiota of rats with high-fat diet，and to explore the effect of Chinese medicine on the regulation of gut microflora and the restoration of gut-liver axis balance. Method:Seventy male SPF Wistar rats were randomly divided into a normal group of 10 and a model group of 60. Mice in the normal group were fed with normal diet and mice in the model group were fed high-fat diet. After 12 weeks，the model group was randomly divided into 6 groups with 10 animals in each group，namely the model group，Xuezhikang group，Liputuo group，and low，medium and high-dose groups of JQHP. The JQHP low-dose，medium-dose and high-dose rats were intragastrically daministered with 0.4，0.8，1.6 g·kg<sup>-1</sup>，respectively， rats in Liputuo group with Liputuo 2 mg·kg<sup>-1</sup>，rats in Xuezhikang group with Xuezhikang 0.1 g·kg<sup>-1</sup>. The rats in the normal group and the model group were intragastrically administered with the same amount of distilled water. Stool were collected after continuous gavaging for 8 weeks，16S rRNA gene sequencing was performed，blood was collected from the abdominal aorta to detect blood lipids，and the liver tissue and ileum tissue were collected for hematoxylin and eosin （HE） staining for pathomorphological observation. Result:Compared with the normal group，the total cholesterol （TC），triglyceride （TG），and low-density lipoprotein （LDL-C） in the model group were significantly increased，while the high-density lipoprotein （HDL-C） was decreased （<italic>P</italic><0.01）. Compared with the model group，TC and TG values were decreased significantly in Xuezhikang group （<italic>P</italic><0.01），HDL-C value was increased （<italic>P</italic><0.05），and in the Liputuo group TC and TG were decreased significantly （<italic>P</italic><0.01）. Compared with the model group，the middle-dose group of JQHP had a certain alleviating effect on liver steatosis and could reduce the infiltration of inflammatory cells. The JQHP could improve the proliferation of lymphoid tissues in the ileal structure，and the middle-dose group has the most significant effect. The results of Shannon curve showed that compared with the normal group，the middle-dose group of JQHP increased significantly （<italic>P</italic><0.01）. Compared with the model group，the middle and high-dose group of JQHP increased （<italic>P</italic><0.05，<italic>P</italic><0.01）. Compared with the middle-dose group of JQHP，the other drug group decreased （<italic>P</italic><0.05，<italic>P</italic><0.01）. Principal component diversity analysis（PCA） showed that the diversity and abundance in the middle-dose JQHP group were higher than those in other drug groups. In linear discriminant analysis（LDA），compared with the normal group，Bacteroidia，Ruminococcaceae，<italic>Bacteroides </italic>S24-7，and <italic>Rumenococcus </italic>UCG-005 were down-regulated in the model group（<italic>P</italic><0.01），while the orders of Desulfovibrionales，Erysipelotrichales and<italic> </italic>Lachnospiraceae were up-regulated in the model group （<italic>P</italic><0.05，<italic>P</italic><0.01）. Compared with the model group，the Bacteroidia，Ruminococcaceae，<italic>Bacteroides</italic> S24-7，and <italic>Rumencoccus</italic> UCG-005 in the middle-dose JQHP group were increased （<italic>P</italic><0.05，<italic>P</italic><0.01），and the orders of Erysipelotrichales were decreased （<italic>P</italic><0.01）. Compared with the middle-dose JQHP group，Bacteroidia，Ruminococcaceae，<italic>Bacteroides</italic> S24-7，and <italic>Rumencoccus</italic> UCG-005 in other drug groups were reduced（<italic>P</italic><0.05，<italic>P</italic><0.01），and the order of Erysipelotrichales and Lachnospiraceae were increased （<italic>P</italic><0.05，<italic>P</italic><0.01）. Conclusion:JQHP can regulate the abundance and diversity of the gut microbiota，improve the state of liver tissue and ileum mucosa，regulate blood lipid levels，and restore the normal intestinal ecological environment. It may be related to the regulation of inflammation-related gut microbiota in order to restore the balance of the gut-liver axis，and the middle-dose JQHP group has the best effect.
ABSTRACT
<p><b>OBJECTIVE</b>To differentiate patients with esophageal cancer or premalignant lesions from the high-risk population for preliminary screening of esophageal cancer using a feature index determined by a computer-aided tongue information acquisition and processing system (DS01-B).</p><p><b>METHODS</b>Totally, 213 patients diagnosed with esophageal cancer or premalignant lesions and 2,840 normal subjects were collected including primarily screened and reexamined, all of them were confirmed with histological examinations. Their tongue color space values and manifestation features were extracted by DS01-B and analyzed. Firstly, the analysis of variance was performed to differentiate normal subjects from patients with esophageal cancer and premalignant lesions. Secondly, the logistic regression was conducted using 10 features and gender, age to get a predictive equation of the possibility of esophageal cancer or premalignant lesions. Lastly, the equation was tested by subjects undergoing primary screening.</p><p><b>RESULTS</b>Saturation (S) values in the HSV color space showed significant differences between patients with esophageal cancer and normal subjects or those with mild atypical hyperplasia (P<0.05); blue-to-yellow (b) values in the Lab color space showed significant differences between patients with esophageal cancer or premalignant lesions and normal subjects (P<0.05). Logistic regression analysis showed that the computer-aided tongue inspection approach had an accuracy of 72.3% (2008/2776) in identifying patients with esophageal cancer or premalignant lesions for preliminary screening in high-risk population.</p><p><b>CONCLUSION</b>Computer-aided tongue inspection, with descriptive and quantitative profile as described in this study, could be applied as a cost- and timeefficient, non-invasive approach for preliminary screening of esophageal cancer in high-risk population.</p>
ABSTRACT
Thoracic obstruction is mainly attributed to the scope of coronary heart disease in modern medicine, and traditional Chinese medicine(TCM) shows a significant effect in the treatment of thoracic obstruction. In this research, a network pharmacology method was carried out to systemically study the underlying mechanism of the core herbal compatibility in TCM on the thoracic obstruction. First, we collected the literature about TCM prescriptions for treating thoracic obstruction from CNKI. Then, a prescription database was establish by TCM inheritance support platform system(V2.5) to determine the medication rules and core herbal compatibility in TCM. Finally, to obtain the potential signaling pathways, KEGG pathway analysis was performed by BATMAN-TCM online analysis tool. Results showed that the potential signal pathway of core herbal compatibility in TCM for the clinical treatment of thoracic obstruction was calcium ion and cGMP-PKG signaling pathway. This study provided a new research strategy for the study of the medication rules and mechanism of traditional Chinese medicine in the treatment of thoracic obstruction.